Researchers at Queen’s University in Kingston, Ontario, Canada, are developing an alternative to nickel-nitrilotriacetic acid (Ni-NTA) to purify recombinant proteins. Because it uses common ...
In response to the letter, "A better way to His-tag," 1 I feel obliged to clear up any possible misunderstandings and allay the concerns of protein researchers currently using or planning to use ...
Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...
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